LOCUS       pLV-[hsa-miR-368]        9119 bp    DNA     circular      1-MAY-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7041..7901
                     /label=Amp(R)
     promoter        8102..8642
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5646..5880
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6455..6910)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5952..6082
                     /label=SV40 pA
     misc_feature    6083..6296
                     /label=SV40 ori
     misc_feature    4967..5563
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3585
                     /label=intron
     CDS             3632..4957
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3375
                     /label=hsa-mir-368 precursor
BASE COUNT     2165 a      2364 c      2464 g      2127 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccg ggaggcccct 
     3121 gccgactgca cctatcctgt gctgtcttta attttgatag attgtgctta ggttcatgct 
     3181 ttccaggact caatccttct ttggtattta aaaggtggat attccttcta tgtttatgtt 
     3241 atttatggtt aaacatagag gaaattccac gttttcagta tcaaatgctg cttggaaaca 
     3301 ttcctggact gaaaccacca tcagggaaga gacaataaag tccccccccc cactccaggg 
     3361 taagtggaaa gtcgggctag ctcgagcttt tggagtacgt cgtctttagg ttggggggag 
     3421 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct 
     3481 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt 
     3541 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaaaacta 
     3601 cccctctaga gtcgagctac cggtcgccac catggtgagc aagggcgagg aggataacat 
     3661 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca 
     3721 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa 
     3781 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt 
     3841 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct 
     3901 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt 
     3961 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg 
     4021 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc 
     4081 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct 
     4141 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa 
     4201 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa 
     4261 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg 
     4321 catggacgag ctgtacaagg acccaccggt cgccaccatg accgagtaca agcccacggt 
     4381 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc 
     4441 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga 
     4501 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga 
     4561 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt 
     4621 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca 
     4681 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt 
     4741 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt 
     4801 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct 
     4861 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgagtgcc cgaaggaccg 
     4921 cgcgacctgg tgcatgaccc gcaagcccgg tgcctgagcg gccgcgtcga caatcaacct 
     4981 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 
     5041 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 
     5101 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 
     5161 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 
     5221 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 
     5281 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 
     5341 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt 
     5401 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 
     5461 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc 
     5521 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattcg agctcggtac 
     5581 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg 
     5641 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg 
     5701 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 
     5761 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 
     5821 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 
     5881 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag 
     5941 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 
     6001 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 
     6061 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc 
     6121 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 
     6181 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 
     6241 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg 
     6301 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt 
     6361 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct 
     6421 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc 
     6481 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 
     6541 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 
     6601 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 
     6661 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 
     6721 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 
     6781 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 
     6841 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg 
     6901 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 
     6961 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 
     7021 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 
     7081 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 
     7141 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 
     7201 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 
     7261 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 
     7321 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 
     7381 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 
     7441 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 
     7501 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 
     7561 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 
     7621 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 
     7681 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 
     7741 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 
     7801 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 
     7861 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 
     7921 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 
     7981 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 
     8041 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 
     8101 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 
     8161 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 
     8221 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 
     8281 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 
     8341 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 
     8401 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 
     8461 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 
     8521 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 
     8581 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 
     8641 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 
     8701 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 
     8761 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 
     8821 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 
     8881 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 
     8941 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 
     9001 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 
     9061 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc 
//
